Anti-BTK Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

PB0104

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of BTK using anti-BTK antibody (PB0104).
Lane 1: recombinant Human BTK Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BTK antigen affinity purified polyclonal antibody (PB0104) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BTK at approximately 36 kDa.
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Figure 2. Western blot analysis of BTK using anti-BTK antibody (PB0104). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HEPG2 whole cell lysates,
Lane 2: K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BTK antigen affinity purified polyclonal antibody (PB0104) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BTK at approximately 76 kDa. The expected band size for BTK is at 76 kDa.
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Figure 3. IHC analysis of BTK using anti-BTK antibody (PB0104).
BTK was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BTK Antibody (PB0104) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 4. IHC analysis of BTK using anti-BTK antibody (PB0104).
BTK was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BTK Antibody (PB0104) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 5. IHC analysis of BTK using anti-BTK antibody (PB0104).
BTK was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BTK Antibody (PB0104) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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产品简介>

产品名称

Anti-BTK Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

AGMX1; AT; ATK; B cell progenitor kinase; BPK; Bruton tyrosine kinase; BTK; IMD1; PSCTK1; Tyrosine protein kinase BTK; XLA

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

BTK

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human BTK recombinant protein (Position: A2-P340). Human BTK shares 98% amino acid (aa) sequence identity with mouse BTK.

计算分子量

76 kDa

实际分子量

76 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

BTK, also known as Bruton's tyrosine kinase, is an enzyme that in humans is encoded by the BTK gene. It is mapped to Xq22.1. BTK plays a crucial role in B cell maturation as well as mast cell activation through the high-affinity IgE receptor. BTK contains a PH domain that binds phosphatidylinositol (3,4,5)-trisphosphate (PIP3). PIP3 binding induces BTK to phosphorylate phospholipase C, which in turn hydrolyzes PIP2, a phosphatidylinositol, into two second messengers, inositol triphosphate (IP3) and diacylglycerol (DAG), which then go on to modulate the activity of downstream proteins during B-cell signalling. This gene also regulates both TLR9 activation and expression in B lymphocytes and is necessary for inhibitory cytokine expression.

Uniprot ID

Q06187

文献引用格式

BTK Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0104)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Bruton agammaglobulinemia tyrosine kinase

蛋白名全称

Tyrosine-protein kinase BTK

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实验步骤

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