Figure 1. Western blot analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (M00555-6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat tissue lysates,
Lane 2: Raji tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD45/PTPRC antigen affinity purified monoclonal antibody (M00555-6) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD45/PTPRC at approximately 180-250 kDa. The expected band size for CD45/PTPRC is at 147 kDa.
Figure 2. IF analysis using anti- CD45/PTPRC Antibody (M00555-6). detected in paraffin-embedded section of human tonsli tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
all(4) 
Figure 3. Flow Cytometry analysis of Raji cells using anti-CD45/PTPRC antibody (M00555-6).
Overlay histogram showing Raji cells stained with M00555-6 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-CD45/PTPRC Antibody (M00555-6) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (M00555-6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat tissue lysates,
Lane 2: Raji tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD45/PTPRC antigen affinity purified monoclonal antibody (M00555-6) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD45/PTPRC at approximately 180-250 kDa. The expected band size for CD45/PTPRC is at 147 kDa.
Figure 2. IF analysis using anti- CD45/PTPRC Antibody (M00555-6). detected in paraffin-embedded section of human tonsli tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 3. Flow Cytometry analysis of Raji cells using anti-CD45/PTPRC antibody (M00555-6).
Overlay histogram showing Raji cells stained with M00555-6 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-CD45/PTPRC Antibody (M00555-6) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 1. Western blot analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (M00555-6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat tissue lysates,
Lane 2: Raji tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD45/PTPRC antigen affinity purified monoclonal antibody (M00555-6) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD45/PTPRC at approximately 180-250 kDa. The expected band size for CD45/PTPRC is at 147 kDa.
Figure 2. IF analysis using anti- CD45/PTPRC Antibody (M00555-6). detected in paraffin-embedded section of human tonsli tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 3. Flow Cytometry analysis of Raji cells using anti-CD45/PTPRC antibody (M00555-6).
Overlay histogram showing Raji cells stained with M00555-6 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-CD45/PTPRC Antibody (M00555-6) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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