Anti-CD45/PTPRC Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM

PB9785

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (PB9785). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD45/PTPRC antigen affinity purified polyclonal antibody (PB9785) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD45/PTPRC at approximately 180-250 kDa. The expected band size for CD45/PTPRC is at 147 kDa.
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IHC analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (PB9785) .
CD45/PTPRC was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD45/PTPRC Antibody (PB9785) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (PB9785).
CD45/PTPRC was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD45/PTPRC Antibody (PB9785) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of Jurkat cells using anti-CD45/PTPRC antibody (PB9785).
Overlay histogram showing Jurkat cells stained with PB9785 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD45/PTPRC Antibody (PB9785) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介>

产品名称

Anti-CD45/PTPRC Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

B220; CD45; CD45R; GP180; L CA; LCA; Leukocyte common antigen; LY5; PTPRC; RPTPC; T200

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, IF, FCM

基因名称

PTPRC

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human CD45, different from the related mouse sequence by eight amino acids, and from the related rat sequence by ten amino acids.

计算分子量

147 kDa

实际分子量

180-250 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene. It is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase. The cytogenetic location of CD45 is 1q31.3-q32.1. This gene is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. In addition, CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

Uniprot ID

P08575

文献引用格式

CD45/PTPRC Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9785)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

protein tyrosine phosphatase, receptor type, C

蛋白名全称

Receptor-type tyrosine-protein phosphatase C

实验方案>

实验步骤

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