Anti-CD45/PTPRC Antibody (Clone#OTI1H2)

Mouse monoclonal antibody

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筛选器： All FCM WB

M00555-7

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Flow cytometric Analysis of living Jurkat cells, using anti-PTPRC antibody, (Red), compared to IgG isotype control, (green) and PBS, (blue).
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HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human PTPRC produced in HEK293T cell (5 ug, right lane)were separated by SDS-PAGE and immunoblotted with anti-PTPRC.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
all(3)

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产品名称

Anti-CD45/PTPRC Antibody (Clone#OTI1H2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

B220; CD45; CD45R; GP180; L CA; LCA; Leukocyte common antigen; LY5; PTPRC; RPTPC; T200

产品类型

Monoclonal

检验物种

human

应用范围

FCM, WB

基因名称

PTPRC

克隆号

OTI1H2

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human PTPRC(NP_002829) produced in HEK293T cell.

计算分子量

145 kDa

实际分子量

147.1 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Uniprot ID

P06800

文献引用格式

CD45/PTPRC Antibody (Clone#OTI1H2) (Boster Biological Technology, Wuhan, China. Catalog#M00555-7)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

protein tyrosine phosphatase, receptor type, C

蛋白名全称

Receptor-type tyrosine-protein phosphatase C

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实验步骤

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