Western blot (WB): | 1:1000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50~100 |
Flow cytometry (FCM): | 1:100 |
Immunofluorescent staining of HepG2 cells using anti-PRL mouse monoclonal antibody.
Anti-PRL mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRL .
Flow cytometric Analysis of Jurkat cells, using anti-PRL antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL.
Immunofluorescent staining of HepG2 cells using anti-PRL mouse monoclonal antibody.
Anti-PRL mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRL .
Flow cytometric Analysis of Jurkat cells, using anti-PRL antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL.