Anti-PKC Alpha/PRKCA (Phospho-T638) Antibody (Clone#OTI3D2)

Mouse monoclonal antibody

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筛选器： All WB

M00743-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-PKC Alpha/PRKCA antibody (M00743-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87 MG whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-PKC Alpha/PRKCA antigen affinity purified monoclonal antibody (M00743-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Alpha/PRKCA at approximately 77 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.
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产品名称

Anti-PKC Alpha/PRKCA (Phospho-T638) Antibody (Clone#OTI3D2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AAG6; PKC A; PKC alpha; PKCA; PKCα; PRKACA; PRKCA; Protein kinase C alpha type; protein kinase C; alpha

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

PRKCA

克隆号

OTI3D2

宿主

Mouse

抗体亚型

IgG2a

免疫原

Human recombinant protein fragment corresponding to amino acids 350-672 of human PRKCA (NP_002728) produced in E.coli.

计算分子量

77 kDa

实际分子量

77 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.

背景资料

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008].

Uniprot ID

P17252

文献引用格式

PKC Alpha/PRKCA (Phospho-T638) Antibody (Clone#OTI3D2) (Boster Biological Technology, Wuhan, China. Catalog#M00743-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Protein kinase C alpha type

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实验步骤

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