Anti-PKC Alpha/PRKCA Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of anti-PKC Alpha/PRKCA antibody (A00743-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates,
  Lane 2: human 293T cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human K562 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified polyclonal antibody (A00743-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Alpha/PRKCA at approximately 80 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.

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  Flow Cytometry analysis of SiHa cells using anti-PKC Alpha/PRKCA antibody (A00743-1).
  Overlay histogram showing SiHa cells stained with A00743-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC Alpha/PRKCA Antibody (A00743-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  Western blot analysis of anti-PKC Alpha/PRKCA antibody (A00743-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates,
  Lane 2: human 293T cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human K562 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified polyclonal antibody (A00743-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Alpha/PRKCA at approximately 80 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.

• 

  Flow Cytometry analysis of SiHa cells using anti-PKC Alpha/PRKCA antibody (A00743-1).
  Overlay histogram showing SiHa cells stained with A00743-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC Alpha/PRKCA Antibody (A00743-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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