Anti-PKC Alpha/PRKCA Antibody (Clone#CHO-16)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4238

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-PKC Alpha/PRKCA antibody (BM4238). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified monoclonal antibody (BM4238) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Alpha/PRKCA at approximately 77 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.
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Immunohistochemical analysis of paraffin-embedded Human thymoma, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded human colon, using PKC alpha Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of Hela cells, using PKC alpha Antibody .
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PKC Alpha/PRKCA Antibody (Clone#CHO-16)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AAG6; PKC A; PKC alpha; PKCA; PKCα; PRKACA; PRKCA; Protein kinase C alpha type; protein kinase C; alpha

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

PRKCA

克隆号

CHO-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PKC alpha

计算分子量

77 kDa

实际分子量

77 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PRKCA1 regulates cardiac contractility and propensity toward heart failure.

Uniprot ID

P17252

文献引用格式

PKC Alpha/PRKCA Antibody (Clone#CHO-16) (Boster Biological Technology, Wuhan, China. Catalog#BM4238)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Protein kinase C alpha type

实验方案>

实验步骤

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