Anti-CDC6 Antibody (Clone#OTI7B12)

Mouse monoclonal antibody

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筛选器： All IHC WB

M01355-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDC6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M01355-2) (1:150)
all(2)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDC6 (1:2000).
all(2)

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产品简介>

产品名称

Anti-CDC6 Antibody (Clone#OTI7B12)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cdc18 related protein; CDC18L; CDC6; CDC6 related protein; HsCDC18; HsCDC6; p62(cdc6)

产品类型

Monoclonal

检验物种

human

应用范围

IHC, WB

基因名称

CDC6

克隆号

OTI7B12

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human CDC6(NP_001245) produced in E.coli.

计算分子量

63 kDa

实际分子量

63 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Uniprot ID

Q99741

文献引用格式

CDC6 Antibody (Clone#OTI7B12) (Boster Biological Technology, Wuhan, China. Catalog#M01355-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cell division cycle 6

蛋白名全称

Cell division control protein 6 homolog

实验方案>

实验步骤

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