Anti-CDC6 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9531

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CDC6 using anti-CDC6 antibody (PB9531). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: JURKAT whole cell lysates,
Lane 3: MM231 whole cell lysates,
Lane 4: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDC6 antigen affinity purified polyclonal antibody (PB9531) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDC6 at approximately 63 kDa. The expected band size for CDC6 is at 63 kDa.
all(3)
IHC analysis of CDC6 using anti-CDC6 antibody (PB9531).
CDC6 was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDC6 Antibody (PB9531) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of CDC6 using anti-CDC6 antibody (PB9531).
CDC6 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDC6 Antibody (PB9531) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-CDC6 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cdc18 related protein; CDC18L; CDC6; CDC6 related protein; HsCDC18; HsCDC6; p62(cdc6)

产品类型

Polyclonal

检验物种

human, rat

应用范围

WB, IHC

基因名称

CDC6

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Cdc6 recombinant protein (Position: V147-A549). Human Cdc6 shares 87.3% amino acid (aa) sequence identity with mouse Cdc6.

计算分子量

63 kDa

实际分子量

63 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cell division control ptotein 6 homolog, also called CDC18L or CDC6, is a protein that in humans is encoded by the CDC6 gene. By analysis of somatic cell hybrids and by fluorescence in situ hybridization, this gene is mapped to 17q21.2. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. And this protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. This gene is involved in the initiation of DNA replication.

Uniprot ID

Q99741

文献引用格式

CDC6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9531)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cell division cycle 6

蛋白名全称

Cell division control protein 6 homolog

实验方案>

实验步骤

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