Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: Caco-2 whole cell lysates,
Lane 6: HepG2 whole cell lysates,
Lane 7: THP-1 whole cell lysates,
Lane 8: PANC-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AIF/AIFM1 antigen affinity purified monoclonal antibody (M01571-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AIF/AIFM1 at approximately 67 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Mouse spleen lysates,
Lane 4: Mouse testicular tissue lysates,
Lane 5: Mouse kidney tissue lysates,
Lane 6: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AIF/AIFM1 antigen affinity purified monoclonal antibody (M01571-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AIF/AIFM1 at approximately 67 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).
all(8) 
Flow Cytometry analysis of Raji cells using anti-AIF/AIFM1 antibody (M01571-1).
Overlay histogram showing Raji cells stained with M01571-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: Caco-2 whole cell lysates,
Lane 6: HepG2 whole cell lysates,
Lane 7: THP-1 whole cell lysates,
Lane 8: PANC-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AIF/AIFM1 antigen affinity purified monoclonal antibody (M01571-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AIF/AIFM1 at approximately 67 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Mouse spleen lysates,
Lane 4: Mouse testicular tissue lysates,
Lane 5: Mouse kidney tissue lysates,
Lane 6: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AIF/AIFM1 antigen affinity purified monoclonal antibody (M01571-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AIF/AIFM1 at approximately 67 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).
Flow Cytometry analysis of Raji cells using anti-AIF/AIFM1 antibody (M01571-1).
Overlay histogram showing Raji cells stained with M01571-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: Caco-2 whole cell lysates,
Lane 6: HepG2 whole cell lysates,
Lane 7: THP-1 whole cell lysates,
Lane 8: PANC-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AIF/AIFM1 antigen affinity purified monoclonal antibody (M01571-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AIF/AIFM1 at approximately 67 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Mouse spleen lysates,
Lane 4: Mouse testicular tissue lysates,
Lane 5: Mouse kidney tissue lysates,
Lane 6: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AIF/AIFM1 antigen affinity purified monoclonal antibody (M01571-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AIF/AIFM1 at approximately 67 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).
Flow Cytometry analysis of Raji cells using anti-AIF/AIFM1 antibody (M01571-1).
Overlay histogram showing Raji cells stained with M01571-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (M01571-1).
AIF/AIFM1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with mouse anti-AIF/AIFM1 Antibody (M01571-1) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
