Anti-AIF/AIFM1 Antibody (Clone#OTI4E6)

Mouse monoclonal antibody

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筛选器： All IHC WB

M01571-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01571-3) (1:500)
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and AIFM1-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-AIFM1 monoclonal antibody M01571-3, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([M01263-6]) as a loading control.
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01571-3) (1:500)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01571-3) (1:500)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01571-3) (1:500)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01571-3) (1:500)
all(11)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01571-3) (1:500)
all(11)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01571-3) (1:500)
all(11)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01571-3) (1:500)
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Western blot analysis of extracts (35ug) from 6 different cell lines and mouse brain tissue lysate by using anti-AIFM1 monoclonal antibody (1:500).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AIFM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AIFM1 (Cat# M01571-3)(1:2000).
all(11)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-AIF/AIFM1 Antibody (Clone#OTI4E6)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AIF; AIFM1; Mitochondrion Marker; PDCD8

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

AIFM1

克隆号

OTI4E6

宿主

Mouse

抗体亚型

IgG2a

免疫原

Human recombinant protein fragment corresponding to amino acids 134-483 of human AIFM1 (NP_001124318) produced in E.coli.

计算分子量

67 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.

Uniprot ID

O95831

文献引用格式

AIF/AIFM1 Antibody (Clone#OTI4E6) (Boster Biological Technology, Wuhan, China. Catalog#M01571-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

apoptosis-inducing factor, mitochondrion-associated, 1

蛋白名全称

Apoptosis-inducing factor 1, mitochondrial

实验方案>

实验步骤

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