Anti-AIF/AIFM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

PB9366

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PB9366). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: human 293T whole cell lysates,
Lane 8: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AIF/AIFM1 antigen affinity purified polyclonal antibody (PB9366) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AIF/AIFM1 at approximately 67 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
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Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PB9366). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat stomach tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat NRK whole cell lysates,
Lane 4: mouse stomach tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse pancrease tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AIF/AIFM1 antigen affinity purified polyclonal antibody (PB9366) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AIF/AIFM1 at approximately 67 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
all(9)
Western blot analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PB9366). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat NRK whole cell lysates,
Lane 3: mouse stomach tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse pancreas tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AIF/AIFM1 antigen affinity purified polyclonal antibody (PB9366) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150). A specific band was detected for AIF/AIFM1 at approximately 70 kDa. The expected band size for AIF/AIFM1 is at 67 kDa.
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IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PB9366).
AIF/AIFM1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AIF/AIFM1 Antibody (PB9366) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PB9366).
AIF/AIFM1 was detected in a paraffin-embedded section of Rat Cardiac Muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AIF/AIFM1 Antibody (PB9366) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PB9366).
AIF/AIFM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AIF/AIFM1 Antibody (PB9366) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PB9366).
AIF/AIFM1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-AIF/AIFM1 Antibody (PB9366) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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IF analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PB9366).
AIF/AIFM1 was detected in an immunocytochemical section of NIH/3T3 cells. The section was incubated with rabbit anti-AIF/AIFM1 Antibody (PB9366) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow cytometry analysis of Hela cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-AIF/AIFM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AIF; AIFM1; Mitochondrion Marker; PDCD8

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

AIFM1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human AIF, identical to the related mouse and rat sequences.

计算分子量

67 kDa

实际分子量

67 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.

Uniprot ID

O95831

文献引用格式

AIF/AIFM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9366)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

apoptosis-inducing factor, mitochondrion-associated, 1

蛋白名全称

Apoptosis-inducing factor 1, mitochondrial

实验方案>

实验步骤

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