Figure 1. Western blot analysis of anti-Cytochrome c/CYCS antibody (M03529-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (M03529-5) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 2. Western blot analysis of anti-Cytochrome c/CYCS antibody (M03529-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse testis tissue lysates,
Lane 9: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (M03529-5) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 3. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in an immunocytochemical section of MCF-7 cells. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of A431 cells using anti-Cytochrome c/CYCS antibody (M03529-5).
Overlay histogram showing A431 cells stained with M03529-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti-Cytochrome c/CYCS antibody (M03529-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (M03529-5) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 2. Western blot analysis of anti-Cytochrome c/CYCS antibody (M03529-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse testis tissue lysates,
Lane 9: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (M03529-5) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 3. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in an immunocytochemical section of MCF-7 cells. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of A431 cells using anti-Cytochrome c/CYCS antibody (M03529-5).
Overlay histogram showing A431 cells stained with M03529-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 1. Western blot analysis of anti-Cytochrome c/CYCS antibody (M03529-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (M03529-5) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 2. Western blot analysis of anti-Cytochrome c/CYCS antibody (M03529-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse testis tissue lysates,
Lane 9: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (M03529-5) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 3. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (M03529-5).
Cytochrome c/CYCS was detected in an immunocytochemical section of MCF-7 cells. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of A431 cells using anti-Cytochrome c/CYCS antibody (M03529-5).
Overlay histogram showing A431 cells stained with M03529-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome c/CYCS Antibody (M03529-5) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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