Figure 1. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human U-937 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human T-47D whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (A03529) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 2. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (A03529) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 3. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human U-937 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human T-47D whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (A03529) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 2. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (A03529) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 3. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 1. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human U-937 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human T-47D whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (A03529) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 2. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (A03529) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
Figure 3. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (A03529).
Cytochrome c/CYCS was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytochrome c/CYCS Antibody (A03529) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
成功添加到购物车
微信客服
微信扫一扫立即咨询
