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Anti-Cytochrome c/CYCS Antibody (Clone#GOC-3)

Rabbit monoclonal antibody

说明书

筛选器: All WB IHC ICC/IF IP

BM4571

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 1. Western blot analysis of anti-Cytochrome c/CYCS antibody (BM4571). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human HepG2 whole cell lysates,
    Lane 4: human HT-1080 whole cell lysates,
    Lane 5: rat kidney tissue lysates,
    Lane 6: rat skeletal muscle tissue lysates,
    Lane 7: mouse kidney tissue lysates,
    Lane 8: mouse skeletal muscle tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified monoclonal antibody (BM4571) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.

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  • Immunohistochemical analysis of paraffin-embedded human liver, using Cytochrome C Antibody.

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  • Immunofluorescent analysis using the Antibody.

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  • Immunofluorescent analysis using the Antibody.

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  • Immunofluorescent analysis using the Antibody.

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  • Immunofluorescent analysis of Hela cells, using Cytochrome C Antibody.

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产品名称
Anti-Cytochrome c/CYCS Antibody (Clone#GOC-3)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CYC; CYCS; Cytochrome c; cytochrome c; somatic; HCS; THC4
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, IP
基因名称
CYCS
克隆号
GOC-3
抗体来源
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human Cytochrome C
计算分子量
12 kDa
实际分子量
14 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Uniprot ID
P99999  
文献引用格式
Cytochrome c/CYCS Antibody (Clone#GOC-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4571)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
Cytochrome c
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:30

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    Anti-Cytochrome c/CYCS Antibody (Clone#GOC-3)

    筛选器: All WB IHC ICC/IF IP

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