Figure 1. Western blot analysis of anti-EIF2A/EIF2S1 antibody (M04387-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-EIF2A/EIF2S1 antigen affinity purified monoclonal antibody (M04387-5) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF2A/EIF2S1 at approximately 36 kDa. The expected band size for EIF2A/EIF2S1 is at 36 kDa.
Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04387-5)
all(3) 
Figure 3. Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
all(3) | Western blot (WB): | 1:2000 |
| Immunohistochemistry (IHC): | 1:150 |
| Flow cytometry (FCM): | 1:100 |
Figure 1. Western blot analysis of anti-EIF2A/EIF2S1 antibody (M04387-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-EIF2A/EIF2S1 antigen affinity purified monoclonal antibody (M04387-5) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF2A/EIF2S1 at approximately 36 kDa. The expected band size for EIF2A/EIF2S1 is at 36 kDa.
Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04387-5)
Figure 3. Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Figure 1. Western blot analysis of anti-EIF2A/EIF2S1 antibody (M04387-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-EIF2A/EIF2S1 antigen affinity purified monoclonal antibody (M04387-5) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF2A/EIF2S1 at approximately 36 kDa. The expected band size for EIF2A/EIF2S1 is at 36 kDa.
Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04387-5)
Figure 3. Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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