Anti-EIF2A/EIF2S1 (Phospho-S51) Antibody (Clone#IO-5)

Rabbit Monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF FCM

BM3942

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-EIF2A/EIF2S1 antibody (BM3942). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EIF2A/EIF2S1 antigen affinity purified monoclonal antibody (BM3942) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF2A/EIF2S1 at approximately 36 kDa. The expected band size for EIF2A/EIF2S1 is at 36 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Phospho-eIF2 alpha (Ser51) Antibody.
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Immunofluorescent analysis using the Antibody.
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产品简介>

产品名称

Anti-EIF2A/EIF2S1 (Phospho-S51) Antibody (Clone#IO-5)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

EIF 2; eIF 2 alpha; EIF 2A; EIF 2alpha; EIF2; EIF2A; EIF2S1/EIF2A; eIF2α

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

EIF2S1

克隆号

IO-5

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-EIF2S1 (S51)

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

EIF2S1(Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz(2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence(GRCh37). Ernst et al.(1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al.(1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.

Uniprot ID

P05198

文献引用格式

EIF2A/EIF2S1 (Phospho-S51) Antibody (Clone#IO-5) (Boster Biological Technology, Wuhan, China. Catalog#BM3942)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Eukaryotic translation initiation factor 2 subunit 1

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