Anti-EIF2A/EIF2S1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

BA3268

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of EIF2A/EIF2S1 using anti-EIF2A/EIF2S1 antibody (BA3268). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: COLO320 whole cell lysates,
Lane 2: CEM whole cell lysates,
Lane 3: RAJI whole cell lysates,
Lane 4: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EIF2A/EIF2S1 antigen affinity purified polyclonal antibody (BA3268) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF2A/EIF2S1 at approximately 36 kDa. The expected band size for EIF2A/EIF2S1 is at 36 kDa.
all(3)
Figure 2. IHC analysis of EIF2A/EIF2S1 using anti-EIF2A/EIF2S1 antibody (BA3268).
EIF2A/EIF2S1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-EIF2A/EIF2S1 Antibody (BA3268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(3)
Figure 3. IHC analysis of EIF2A/EIF2S1 using anti-EIF2A/EIF2S1 antibody (BA3268).
EIF2A/EIF2S1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-EIF2A/EIF2S1 Antibody (BA3268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-EIF2A/EIF2S1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

EIF 2; eIF 2 alpha; EIF 2A; EIF 2alpha; EIF2; EIF2A; EIF2S1/EIF2A; eIF2α

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

EIF2S1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1.

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

EIF2S1(Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz(2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence(GRCh37). Ernst et al.(1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al.(1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.

Uniprot ID

P05198

文献引用格式

EIF2A/EIF2S1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3268)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

蛋白名全称

Eukaryotic translation initiation factor 2 subunit 1

实验方案>

实验步骤

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