Anti-14-3-3 Epsilon/YWHAE Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC FCM ICC/IF ELISA

A01687-4

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- YWHAE antibody (A01687-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human hepg2 whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: human HEK293 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: human A549 whole cell lysates,Lane 8: human Raji whole cell lysates,Lane 9: Rat brain tissue lysates,Lane 10: Mouse brain tissue lysates,Lane 11: Mouse NIH/3T3 whole cell lysates,Lane 12: Mouse RAW264.7 whole cell lysates,Use rabbit anti- YWHAE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAE at approximately 29KD. The expected band size for YWHAE is at 29KD.
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Figure 2. IHC analysis using anti- YWHAE antibody (A01687-4). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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Figure 4. ICC analysis using anti- YWHAE antibody (A01687-4). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-14-3-3 Epsilon/YWHAE Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

14 3 3 protein epsilon; 14 3 3E; 14-3-3; 14-3-3 epsilon; KCIP 1; MDCR; MDS; YWHAE

产品类型

Polyclonal

检验物种

human,mouse,rat

应用范围

WB, IHC, ICC/IF, FCM, ELISA

基因名称

YWHAE

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human YWHAE recombinant protein (Position: M1-Q255).

计算分子量

29 kDa

实际分子量

29 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Uniprot ID

P62258

RRID

AB_3081471

文献引用格式

14-3-3 Epsilon/YWHAE Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01687-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon

蛋白名全称

14-3-3 protein epsilon

实验方案>

实验步骤

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