Figure 1. Western blot analysis of anti- YWHAE Antibody (M01687-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: SH-SY5Y whole cell lysates,
Lane 3: SW620 whole cell lysates,
Lane 4: Raji whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: NIH/3T3 whole cell lysates.
Use mouse anti- YWHAE 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for YWHAE at approximately 29KD. The expected band size for YWHAE is at 29KD.
Figure 2. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(11) 
Figure 3. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(11) 
Figure 4. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(11) 
Figure 5. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of endometrial cance tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(11) 
Figure 6. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(11) 
Figure 7. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(11) 
Figure 8. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(11) 
Figure 10. Flow cytometry analysis of ANA-1 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
all(11) 
Figure 11. Flow cytometry analysis of NRK cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
all(11) 
Figure 9. ICC analysis using anti- YWHAE Antibody (M01687-2). was detected in immersion fixed CACO-2 cell. Cells were stained using the Dylight594-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1141) and counterstained with DAPI (blue).
all(11) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- YWHAE Antibody (M01687-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: SH-SY5Y whole cell lysates,
Lane 3: SW620 whole cell lysates,
Lane 4: Raji whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: NIH/3T3 whole cell lysates.
Use mouse anti- YWHAE 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for YWHAE at approximately 29KD. The expected band size for YWHAE is at 29KD.
Figure 2. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of endometrial cance tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 6. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 7. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 8. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 10. Flow cytometry analysis of ANA-1 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
Figure 11. Flow cytometry analysis of NRK cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
Figure 9. ICC analysis using anti- YWHAE Antibody (M01687-2). was detected in immersion fixed CACO-2 cell. Cells were stained using the Dylight594-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1141) and counterstained with DAPI (blue).
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