Anti-Beta Tubulin/TUBB Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human SiHa whole cell lysates,
  Lane 2: human 293T whole cell lysates,
  Lane 3: human HepG2 whole cell lysates,
  Lane 4: monkey COS-7 whole cell lysates,
  Lane 5: chicken heart tissue lysates,
  Lane 6: rat brain tissue lysates,
  Lane 7: rat PC-12 whole cell lysates,
  Lane 8: mouse brain tissue lysates,
  Lane 9: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Tubulin/TUBB antigen affinity purified polyclonal antibody (A01857-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Tubulin/TUBB at approximately 50 kDa. The expected band size for Beta Tubulin/TUBB is at 50 kDa.

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  Figure 2. IHC analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1) .
  Beta Tubulin/TUBB was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1) .
  Beta Tubulin/TUBB was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IF analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1).
  Beta Tubulin/TUBB was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 5. Flow Cytometry analysis of SiHa cells using anti-Beta Tubulin/TUBB antibody (A01857-1).
  Overlay histogram showing SiHa cells stained with A01857-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.