Anti-Beta Tubulin/TUBB Antibody

筛选器： All FCM WB ICC/IF

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  Figure 3. Flow cytometry analysis of SiHa cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).

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  Figure 1. Western blot analysis of anti- TUBB antibody (M01857-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human HELA whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human HL-60 whole cell lysates,Lane 5: human Raji whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse brain tissue lysates.Use rabbit anti- TUBB 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for TUBB at approximately 55KD. The expected band size for TUBB is at 55KD.

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  Figure 2. ICC analysis using anti- TUBB antibody (M01857-3). was detected in immersion fixed A431 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).