Anti-14-3-3 Sigma/SFN Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC FCM ICC/IF

BA3752

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of Anti-SFN antibody (BA3752). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: HELA whole cell lysates,Lane 2: PC-3 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates,Use rabbit Anti-SFN 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SFN at approximately 28KD. The expected band size for SFN is at 28KD.
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Figure 2. IHC analysis using Anti-SFN antibody (BA3752) detected in paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis using Anti-SFN antibody (BA3752) detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis using Anti-SFN antibody (BA3752) detected in paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 7. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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Figure 5. ICC analysis using anti- SFN antibody (BA3752). was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
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Figure 6. ICC analysis using anti- SFN antibody (BA3752). was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1142) and counterstained with DAPI (blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-14-3-3 Sigma/SFN Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

14 3 3 protein sigma; 14-3-3; 14-3-3 sigma; HME1; SFN; stratifin; YWHAS

产品类型

Polyclonal

检验物种

human,mouse,rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

SFN

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma, identical to the related rat and mouse sequences.

计算分子量

28 kDa

实际分子量

28 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Uniprot ID

P31947

文献引用格式

14-3-3 Sigma/SFN Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3752)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

stratifin

蛋白名全称

14-3-3 protein sigma

实验方案>

实验步骤

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