Anti-14-3-3 Sigma/SFN Antibody

50μl/1180 100μl/1960 150μl/2780

14 3 3 protein sigma; 14-3-3; 14-3-3 sigma; HME1; SFN; stratifin; YWHAS

Monoclonal

human, rat

WB, IHC

SFN

Rabbit

IgG

A synthesized peptide derived from human 14-3-3 sigma

28 kDa

28 kDa

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

Affinity-chromatography

500 ug/ml

Liquid

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

P31947

14-3-3 Sigma/SFN Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM4692)

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

14-3-3 protein sigma

Western blot (WB):	1:500-2000
Immunohistochemistry (IHC):	1:50-200