Human IFN Gamma/IFNG ELISA KIt

96T/2280

IFG; IFI; IFN gamma; IFN γ; IFNG; IFN-gamma; IFN-γ

E.coli, Q24-Q166

There is no detectable cross-reactivity with other relevant proteins.

<1 pg/ml

15.6 pg/ml - 1000 pg/ml

IFNG

interferon, gamma

P01579

Human IFN Gamma/IFNG ELISA KIt (Boster Biological Technology, Wuhan, China. Catalog#EK0373)

Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

20-25分钟

Catalog number	Description	Quantity
EK0373-CAP	96-well plate precoated with anti- human IFN gamma antibody	1
EK0373-ST	Lyophilized recombinant human IFN gamma standard	10ng/tube×2
EK0373-DA	Biotinylated anti- human IFN gamma antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	34	150	469	33	139	437
Standard deviation	1.73	8.7	29.54	1.91	9.73	31.9
CV(%)	5.1	5.8	6.3	5.8	7	7.3

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.011	0.056	0.098	0.183	0.366	0.646	1.382	2.405

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum

3.5h