Mouse IFN Gamma/IFNG ELISA Kit

96T/2280

IFG; IFI; IFN gamma; IFN γ; IFNG; IFN-gamma; IFN-γ

Expression system for standard: E.coli; Immunogen sequence: H23-C155

There is no detectable cross-reactivity with other relevant proteins.

<5pg/ml

31.2pg/ml-2000pg/ml

IFNG

Interferon gamma

P01580

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Catalog number	Description	Quantity
EK0375-CAP	96-well plate precoated with anti- mouse IFN gamma antibody	1
EK0375-ST	Lyophilized recombinant mouse IFN gamma standard	10ng/tube×2
EK0375-DA	Biotinylated anti- mouse IFN gamma antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	68	224	760	70	232	686
Standard deviation	2.92	12.32	33.44	3.08	12.76	30.18
CV(%)	4.3%	5.5%	4.4%	4.4%	5.5%	4.4%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.049	0.142	0.253	0.375	0.595	1.015	1.355	1.997

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum

3.5h