Human IL-1 beta/IL1B ELISA Kit

96T/2280

IL1 beta; Catabolin; IL 1; IL 1 beta; IL1 BETA; IL-1 beta; IL1- beta; IL1B; IL1beta; IL1F2; IL-1β; Interleukin 1 beta; interleukin 1; beta; Pro IL-1β; IL 1B; IL-1B

E.coli,A117-S269

There is no detectable cross-reactivity with other relevant proteins.

<0.15 pg/ml

3.9 pg/ml - 250 pg/ml

IL1B

interleukin 1, beta

P01584

Human IL-1 beta/IL1B ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0392)

Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

15-20分钟

Catalog number	Description	Quantity
EK0392-CAP	96-well plate precoated with anti- human IL-1 beta antibody	1
EK0392-ST	Lyophilized recombinant human IL-1 beta standard	1ng/tubex2
EK0392-DA	Biotinylated anti- human IL-1 beta antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	11	60	123	10	58	112
Standard deviation	0.45	2.88	8.97	0.57	3.24	9.96
CV(%)	4.1	4.8	7.3	5.7	5.6	8.9

Concentration(pg/ml)	0	3.9	7.8	15.6	31.2	62.5	125	250
O.D.	0.003	0.086	0.161	0.292	0.557	1.029	1.617	2.222

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h