Rat IL-1 beta/IL1B ELISA Kit

96T/2480

IL1 beta; Catabolin; IL 1; IL 1 beta; IL1 BETA; IL-1 beta; IL1- beta; IL1B; IL1beta; IL1F2; IL-1β; Interleukin 1 beta; interleukin 1; beta; Pro IL-1β; IL 1B; IL-1B

Expression system for standard: E.coli; Immunogen sequence: V117-S268

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

31.2pg/ml-2000pg/ml

IL1B

interleukin 1, beta

Q63264

Rat IL-1 beta/IL1B ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0393)

The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).

Catalog number	Description	Quantity
EK0393-CAP	96-well plate precoated with anti- rat IL-1 beta antibody	1
EK0393-ST	Lyophilized recombinant rat IL-1 beta standard	10ng/tubex2
EK0393-DA	Biotinylated anti- rat IL-1 beta antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	58	233	962	62	241	881
Standard deviation	3.65	11.41	69.26	4.21	12.29	68.71
CV(%)	6.3%	4.9%	7.2%	6.8%	5.1%	7.8%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.022	0.072	0.136	0.213	0.364	0.779	1.626	2.440

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)