Rat IL6 ELISA Kit

96T/2480

B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; HSF; Hybridoma growth factor; IL 6; IL6; IL-6; Interferon beta 2; Interleukin 6

Expression system for standard: E.coli; Immunogen sequence: F25-T211

There is no detectable cross-reactivity with other relevant proteins.

<5pg/ml

62.5pg/ml-4000pg/ml

IL6

interleukin 6

P20607

Rat IL6 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0412)

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH). Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.

Catalog number	Description	Quantity
EK0412-CAP	Anti-Rat Il6 Pre-coated 96-well strip microplate	1
EK0412-ST	Rat Il6 Standard	10ng/tube×2
EK0412-DA	Rat Il6 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	193	579	1531	210	564	1466
Standard deviation	9.65	35.89	90.32	11.55	40.04	109.95
CV(%)	5%	6.2%	5.9%	5.5%	7.1%	7.5%

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.036	0.082	0.140	0.198	0.373	0.751	1.292	2.032

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)