Mouse IL6 Quick ELISA Kit

96T/3580

B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; HSF; Hybridoma growth factor; IL 6; IL6; IL-6; Interferon beta 2; Interleukin 6

Expression system for standard: E.coli; Immunogen sequence: F25-T211

There is no detectable cross-reactivity with other relevant proteins.

<1/ml

15.6pg/ml-1000pg/ml

IL6

interleukin 6

P05231

Mouse IL6 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0411)

The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

15分钟

Catalog number	Description	Quantity
FEK0411-CAP	Anti-mouse IL-6 Pre-coated 96-well Strip Microplate	1
FEK0411-ST	mouse IL-6 standard	10ng/tube×2
FEK0411-H	HRP Linked Anti-mouse IL-6 Antibody	6ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	10	38	145	9	37	130
Standard deviation	0.62	1.67	10.73	0.64	1.66	11.18
CV(%)	6.2%	4.4%	7.4%	7.2%	4.5%	8.6%

Concentration(pg/ml)	0	4.69	9.38	18.75	37.5	75	150	300
O.D.	0.032	0.110	0.183	0.317	0.588	1.068	1.829	2.434

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)