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Anti-His Tag Antibody

Mouse monoclonal antibody

说明书

筛选器: All WB

M30975-1

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Western blot analysis of different concentration of Anti- His Tag (19b10) Monoclonal Antibody (M30975-1)Blocking: Incubate the membrane with 5% Non-Fat Milk, 1×PBS, 4℃, overnightAntibody Incubation: Diluted antibody in 5% Non-Fat Milk, 1×TBS, 0.1% Tween-20, RT, 30mins.

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产品简介>

产品名称
Anti-His Tag Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
产品类型
Monoclonal
检验物种
All species
应用范围
WB
克隆号
19b10
抗体来源
Mouse
抗体亚型
IgG2b
免疫原
This antibody is produced by immunizing animals with a synthetic His tag peptide corresponding to KLH.
成分
1 mg/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
protein G purified.
浓度
500ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
A Polyhistidine-Tag is an amino acids motif in proteins that consists of at least six histidine(His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-Tag, 6xHis-Tag, His6 Tag and by the trademarked name His-Tag. Polyhistidine-Tags are often used for affinity purification of genetically modified proteins.
文献引用格式
His Tag Antibody (Boster Biological Technology, Wuhan, China. Catalog#M30975-1)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
推荐稀释比
Western blot (WB):1:500-2000

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    Anti-His Tag Antibody

    筛选器: All WB

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