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Anti-VEGF/VEGFA Antibody

Rabbit polyclonal antibody

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筛选器: All WB ELISA

A00045-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 1. Western blot analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (A00045-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat thymus tissue lysates,
    Lane 2: mouse thymus tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGF/VEGFA antigen affinity purified polyclonal antibody (A00045-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGF/VEGFA at approximately 27 kDa. The expected band size for VEGF/VEGFA is at 25 kDa.

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产品名称
Anti-VEGF/VEGFA Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, ELISA
基因名称
Vegfa
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived mouse VEGF/Vegfa recombinant protein (Position: E38-R214).
计算分子量
25 kDa
实际分子量
27 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Vascular endothelial growth factor A (VEGF-A) is a protein that in humans is encoded by the VEGFA gene. It is mapped to 17 C; 17 22.79 cM. This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein,which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells,and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome,also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism,and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame,which is located within an internal ribosome entry site.
Uniprot ID
Q00731  
RRID
文献引用格式
VEGF/VEGFA Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00045-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
vascular endothelial growth factor A
蛋白名全称
Vascular endothelial growth factor A
推荐稀释比
Western blot (WB):1:500-2000
ELISA:1:100-1000

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    Anti-VEGF/VEGFA Antibody

    筛选器: All WB ELISA

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