Anti-VEGF/VEGFA Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA0407

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (BA0407). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGF/VEGFA antigen affinity purified polyclonal antibody (BA0407) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGF/VEGFA at approximately 27 kDa. The expected band size for VEGF/VEGFA is at 27 kDa.
all(3)
Figure 2. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (BA0407) .
VEGF/VEGFA was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (BA0407) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(3)
Figure 3. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (BA0407) .
VEGF/VEGFA was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (BA0407) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-VEGF/VEGFA Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

VEGFA

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human VEGF, identical to the related mouse and rat sequences.

计算分子量

27 kDa

实际分子量

27 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. Vascular endothelial growth factor(VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Vascular endothelial growth factor(VEGF) stimulates neurogenesis in vitro and in vivo.

Uniprot ID

P15692

RRID

AB_2560934

文献引用格式

VEGF/VEGFA Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0407)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

vascular endothelial growth factor A

蛋白名全称

Vascular endothelial growth factor A(VEGF-A)

实验方案>

实验步骤

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