Figure 1. Western blot analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse Hepa1-6 whole cell lysates,
Lane 7: human placenta tissue lysates,
Lane 8: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGF/VEGFA antigen affinity purified polyclonal antibody (PB9071) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGF/VEGFA at approximately 27 kDa. The expected band size for VEGF/VEGFA is at 27 kDa.
Figure 2. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. Flow Cytometry analysis of SiHa cells using anti-VEGF/VEGFA antibody (PB9071).
Overlay histogram showing SiHa cells stained with PB9071 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 23 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse Hepa1-6 whole cell lysates,
Lane 7: human placenta tissue lysates,
Lane 8: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGF/VEGFA antigen affinity purified polyclonal antibody (PB9071) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGF/VEGFA at approximately 27 kDa. The expected band size for VEGF/VEGFA is at 27 kDa.
Figure 2. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. Flow Cytometry analysis of SiHa cells using anti-VEGF/VEGFA antibody (PB9071).
Overlay histogram showing SiHa cells stained with PB9071 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 1. Western blot analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse Hepa1-6 whole cell lysates,
Lane 7: human placenta tissue lysates,
Lane 8: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGF/VEGFA antigen affinity purified polyclonal antibody (PB9071) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGF/VEGFA at approximately 27 kDa. The expected band size for VEGF/VEGFA is at 27 kDa.
Figure 2. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071) .
VEGF/VEGFA was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. Flow Cytometry analysis of SiHa cells using anti-VEGF/VEGFA antibody (PB9071).
Overlay histogram showing SiHa cells stained with PB9071 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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