Anti-VEGF/VEGFA Antibody (Clone#OTI4G3)

Mouse monoclonal antibody

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筛选器： All IHC WB ICC/IF

MA00045

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Figure 1. Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VEGF mouse monoclonal antibody. (MA00045)
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Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VEGF monoclonal antibody.
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Figure 3. HEK293T cells were transfected with the overexpression plasmids of 9 VEGF isoforms (from left to right:VEGF206, ; VEGF189, ; L-VEGF165, ; VEGF165, ; L-VEGF121, ; VEGF iso18, ; VEGF183, ; VEGF145, ; L-VEGF189,) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (1:1000) or anti-VEGFA mouse monoclonal antibody. (MA00045, 1:500)
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Figure 4. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-VEGF mouse monoclonal antibody. (MA00045)
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Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-VEGF/VEGFA Antibody (Clone#OTI4G3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF

产品类型

Monoclonal

检验物种

human, dog

应用范围

IHC, WB, ICC/IF

基因名称

VEGFA

克隆号

OTI4G3

宿主

Mouse

抗体亚型

IgG2b

免疫原

Human recombinant protein fragment corresponding to amino acids 27-233 of human VEGFA (NP_003367) produced in E.coli.

计算分子量

27 kDa

实际分子量

23.8 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

0.46 mg/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Vascular endothelial growth factor A (VEGF-A) is a protein that in humans is encoded by the VEGFA gene. It is mapped to 17 C; 17 22.79 cM. This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

Uniprot ID

P15692

文献引用格式

VEGF/VEGFA Antibody (Clone#OTI4G3) (Boster Biological Technology, Wuhan, China. Catalog#MA00045)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

vascular endothelial growth factor A

蛋白名全称

Vascular endothelial growth factor A

实验方案>

实验步骤

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