Anti-P glycoprotein/ABCB1 Antibody

筛选器： All FCM WB ICC/IF

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  Flow Cytometry analysis of U2OS cells using anti-P glycoprotein/ABCB1 antibody (A00049-1).
  Overlay histogram showing U2OS cells stained with A00049-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P glycoprotein/ABCB1 Antibody (A00049-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of P glycoprotein/ABCB1 using anti-P glycoprotein/ABCB1 antibody (A00049-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human THP-1 whole cell lysates,
  Lane 2: human A375 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P glycoprotein/ABCB1 antigen affinity purified polyclonal antibody (A00049-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P glycoprotein/ABCB1 at approximately 130-180 kDa. The expected band size for P glycoprotein/ABCB1 is at 141 kDa.

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  ICC/IF analysis of P glycoprotein/ABCB1 using anti-P glycoprotein/ABCB1 antibody (A00049-1).
  P glycoprotein/ABCB1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-P glycoprotein/ABCB1 Antibody (A00049-1) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of U2OS cells using anti-P glycoprotein/ABCB1 antibody (A00049-1).
  Overlay histogram showing U2OS cells stained with A00049-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P glycoprotein/ABCB1 Antibody (A00049-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of P glycoprotein/ABCB1 using anti-P glycoprotein/ABCB1 antibody (A00049-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human THP-1 whole cell lysates,
  Lane 2: human A375 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P glycoprotein/ABCB1 antigen affinity purified polyclonal antibody (A00049-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P glycoprotein/ABCB1 at approximately 130-180 kDa. The expected band size for P glycoprotein/ABCB1 is at 141 kDa.

• 

  ICC/IF analysis of P glycoprotein/ABCB1 using anti-P glycoprotein/ABCB1 antibody (A00049-1).
  P glycoprotein/ABCB1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-P glycoprotein/ABCB1 Antibody (A00049-1) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).