Anti-IFN Gamma/IFNG Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC ELISA(Cap)

A00393-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of IFN Gamma/IFNG using anti-IFN Gamma/IFNG antibody (A00393-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: mouse spleen lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IFN Gamma/IFNG antigen affinity purified polyclonal antibody (A00393-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IFN Gamma/IFNG at approximately 19 kDa. The expected band size for IFN Gamma/IFNG is at 19 kDa.
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Figure 2. IHC analysis of IFN Gamma/IFNG using anti-IFN Gamma/IFNG antibody (A00393-3).
IFN Gamma/IFNG was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-IFN Gamma/IFNG Antibody (A00393-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of IFN Gamma/IFNG using anti-IFN Gamma/IFNG antibody (A00393-3).
IFN Gamma/IFNG was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was incubated with rabbit anti-IFN Gamma/IFNG Antibody (A00393-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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产品简介>

产品名称

Anti-IFN Gamma/IFNG Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

IFG; IFI; IFN gamma; IFN γ; IFNG; IFN-gamma; IFN-γ

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA(Cap)

基因名称

IFNG

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Interferon gamma recombinant protein (Position: Q24-G161). Human Interferon gamma shares 40.3% and 36.7% amino acid (aa) sequence identity with mouse and rat Interferon gamma, respectively.

计算分子量

19 kDa

实际分子量

19 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

IInterferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Uniprot ID

P01579

RRID

AB_3081095

文献引用格式

IFN Gamma/IFNG Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00393-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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