Anti-IFN Gamma/IFNG Antibody (Clone#AFIG-9)

筛选器： All WB

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  Figure 1. Western blot analysis of IFN Gamma using anti-IFN Gamma antibody (M00393-4).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
  Lane 1: recombinant human IFN Gamma protein 15ng,
  Lane 2: recombinant human IFN Gamma protein 10ng.
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IFN Gamma antigen affinity purified monoclonal antibody (Catalog # M00393-4) at 0.5 μg/mL overnight at 4～C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

• 

  Figure 1. Western blot analysis of IFN Gamma using anti-IFN Gamma antibody (M00393-4).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
  Lane 1: recombinant human IFN Gamma protein 15ng,
  Lane 2: recombinant human IFN Gamma protein 10ng.
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IFN Gamma antigen affinity purified monoclonal antibody (Catalog # M00393-4) at 0.5 μg/mL overnight at 4～C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.