Anti-IFN Gamma/IFNG Antibody (Clone#AFII-9)

Rat monoclonal antibody

说明书

筛选器： All IHC

M00393-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. IHC analysis of IFN Gamma using anti-IFN Gamma antibody (M00393-5).
IFN Gamma was detected in paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IFN Gamma Antibody (M00393-5) overnight at 4～C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37～C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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产品简介>

产品名称

Anti-IFN Gamma/IFNG Antibody (Clone#AFII-9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

IFG; IFI; IFN gamma; IFN γ; IFNG; IFN-gamma; IFN-γ

产品类型

Monoclonal

检验物种

mouse

应用范围

IHC

基因名称

Ifng

克隆号

AFII-9

抗体来源

Rat

抗体亚型

IgG1, κ

免疫原

Recombinant mouse IFNγ

成分

200 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

200 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Uniprot ID

P01580

文献引用格式

IFN Gamma/IFNG Antibody (Clone#AFII-9) (Boster Biological Technology, Wuhan, China. Catalog#M00393-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interferon gamma

蛋白名全称

Interferon gamma

实验方案>

实验步骤

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