Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of IFN Gamma/IFNG using anti-IFN Gamma/IFNG antibody (PB0062).
Lane 1: recombinant Rat Interferon gamma Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IFN Gamma/IFNG antigen affinity purified polyclonal antibody (PB0062) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IFN Gamma/IFNG at approximately 19 kDa.
Figure 1. Western blot analysis of IFN Gamma/IFNG using anti-IFN Gamma/IFNG antibody (PB0062).
Lane 1: recombinant Rat Interferon gamma Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IFN Gamma/IFNG antigen affinity purified polyclonal antibody (PB0062) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IFN Gamma/IFNG at approximately 19 kDa.