Anti-Vinculin/VCL Antibody

Rabbit polyclonal antibody

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筛选器： All WB

A01207

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat RH-35 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vinculin/VCL antigen affinity purified polyclonal antibody (A01207) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vinculin/VCL at approximately 124 kDa. The expected band size for Vinculin/VCL is at 124 kDa.
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产品简介>

产品名称

Anti-Vinculin/VCL Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CMD1W; Metavinculin; MVCL; VCL; Vinculin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

VCL

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134). Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin.

计算分子量

124 kDa

实际分子量

124 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Uniprot ID

P18206

RRID

AB_3081381

文献引用格式

Vinculin/VCL Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01207)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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