Anti-SNAP25 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM ELISA

A01625

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-SNAP25 antibody (A01625). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (A01625) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SNAP25 at approximately 25 kDa. The expected band size for SNAP25 is at 23 kDa.
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IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SNAP25 Antibody (A01625) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SNAP25 Antibody (A01625) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SNAP25 Antibody (A01625) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SNAP25 Antibody (A01625) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in a paraffin-embedded section of human brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SNAP25 Antibody (A01625) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-SNAP25 Antibody (A01625) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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ICC/IF analysis of SNAP25 using anti-SNAP25 antibody (A01625).
SNAP25 was detected in an immunocytochemical section of SH-SY5Y cells. The section was incubated with rabbit anti-SNAP25 Antibody (A01625) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of U2OS cells using anti-SNAP25 antibody (A01625).
Overlay histogram showing U2OS cells stained with A01625 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (A01625) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-SNAP25 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

RIC 4; RIC4; SEC9; SNAP; SNAP 25; SNAP25; SUP; Super protein

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM, ELISA

基因名称

SNAP25

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human SNAP25 recombinant protein (Position:M1-L203). Human SNAP25 shares 100% amino acid (aa) sequence identity with both mouse and rat SNAP25.

计算分子量

23 kDa

实际分子量

25 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Synaptosome-associated protein of 25,000 daltons, also known as SNAP-25, is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE (the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa (SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E (BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms (a and b) have been described, which are produced by alternative splicing. It is identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

Uniprot ID

P60880

文献引用格式

SNAP25 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01625)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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