Anti-SNAP25 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC

BA1400

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of SNAP25 using anti-SNAP25 antibody (BA1400). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (BA1400) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SNAP25 at approximately 25 kDa. The expected band size for SNAP25 is at 23 kDa.
all(2)
IHC analysis of SNAP25 using anti-SNAP25 antibody (BA1400).
SNAP25 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SNAP25 Antibody (BA1400) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-SNAP25 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

RIC 4; RIC4; SEC9; SNAP; SNAP 25; SNAP25; SUP; Super protein

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

SNAP25

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25, identical to the related rat and mouse sequences.

计算分子量

23 kDa

实际分子量

25 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Synaptosome-associated protein of 25,000 daltons also known as SNAP-25 is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE(the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa(SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E(BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms(a and b) have been described, which are produced by alternative splicing. Nagy et al.(2004) identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

Uniprot ID

P60880

文献引用格式

SNAP25 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1400)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

synaptosomal-associated protein, 25kDa

蛋白名全称

Synaptosomal-associated protein 25(SNAP-25)

实验方案>

实验步骤

引用文献(0)>

清除