Anti-SNAP25 Antibody (Clone#ABHE-19)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IP

BM5167

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-SNAP25 antibody (BM5167). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNAP25 antigen affinity purified monoclonal antibody (BM5167) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SNAP25 at approximately 25 kDa. The expected band size for SNAP25 is at 23 kDa.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-SNAP25 Antibody (Clone#ABHE-19)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

RIC 4; RIC4; SEC9; SNAP; SNAP 25; SNAP25; SUP; Super protein

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, IP

基因名称

SNAP25

克隆号

ABHE-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human SNAP25

计算分子量

23 kDa

实际分子量

25 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Synaptosome-associated protein of 25,000 daltons, also known as SNAP-25, is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE (the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa (SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E (BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms (a and b) have been described, which are produced by alternative splicing. It is identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

Uniprot ID

P60880

文献引用格式

SNAP25 Antibody (Clone#ABHE-19) (Boster Biological Technology, Wuhan, China. Catalog#BM5167)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Synaptosomal-associated protein 25

实验方案>

实验步骤

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