Anti-SNAP25 Antibody (Clone#OTI 1D5)

Mouse monoclonal antibody

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M01625-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody, and then analyzed by flow cytometry.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01625-2)
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Anti-SNAP25 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25 .
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-SNAP25 Antibody (Clone#OTI 1D5)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

RIC 4; RIC4; SEC9; SNAP; SNAP 25; SNAP25; SUP; Super protein

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

SNAP25

克隆号

OTI 1D5

宿主

Mouse

抗体亚型

IgG2a

免疫原

Full length human recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cell.

计算分子量

23 kDa

实际分子量

23.2 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot ID

P60880

文献引用格式

SNAP25 Antibody (Clone#OTI 1D5) (Boster Biological Technology, Wuhan, China. Catalog#M01625-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

synaptosomal-associated protein, 25kDa

蛋白名全称

Synaptosomal-associated protein 25(SNAP-25)

实验方案>

实验步骤

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