Anti-CD8A Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A02236-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD8A using anti-CD8A antibody (A02236-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD8A antigen affinity purified polyclonal antibody (A02236-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD8A at approximately 26 kDa. The expected band size for CD8A is at 27 kDa.
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IHC analysis of CD8A using anti-CD8A antibody (A02236-1).
CD8A was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD8A Antibody (A02236-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD8A using anti-CD8A antibody (A02236-1).
CD8A was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD8A Antibody (A02236-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
Flow Cytometry analysis of EL-4 cells using anti-CD8 alpha antibody (A02236-1).Overlay histogram showing EL-4 cells stained with A02236-1 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介>

产品名称

Anti-CD8A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD8; CD8A; CD8a molecule; Leu2; MAL; p32

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

CD8A

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196). Mouse CD8 shares 47.9% and 62.3% amino acid (aa) sequence identity with human and rat CD8, respectively.

计算分子量

27 kDa

实际分子量

26 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Uniprot ID

P01731

RRID

AB_3081533

文献引用格式

CD8A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02236-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD8 antigen, alpha chain

蛋白名全称

T-cell surface glycoprotein CD8 alpha chain

实验方案>

实验步骤

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