Anti-CD8A Antibody (Clone#EAF-3)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF

BM4379

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-CD8A antibody (BM4379). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD8A antigen affinity purified monoclonal antibody (BM4379) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD8A at approximately 36 kDa. The expected band size for CD8A is at 23 kDa.
all(9)
Immunohistochemical analysis of paraffin-embedded Rat kidney, using the Antibody.
all(9)
Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody.
all(9)
Immunohistochemical analysis of paraffin-embedded Human kidney, using the Antibody.
all(9)
Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody.
all(9)
Immunohistochemical analysis of paraffin-embedded human colon, using CD8 Antibody.
all(9)
Immunofluorescent analysis using the Antibody.
all(9)
Immunofluorescent analysis using the Antibody.
all(9)
Immunofluorescent analysis of Jurkat cells, using CD8 Antibody .
all(9)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD8A Antibody (Clone#EAF-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD8; CD8A; CD8a molecule; Leu2; MAL; p32

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF

基因名称

CD8A

克隆号

EAF-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human CD8

计算分子量

23 kDa

实际分子量

26 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Uniprot ID

P01732

文献引用格式

CD8A Antibody (Clone#EAF-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4379)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

T-cell surface glycoprotein CD8 alpha chain

实验方案>

实验步骤

引用文献(0)>

清除