Anti-CD8A Antibody (Clone#OTI7C10)

Mouse monoclonal antibody

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筛选器： All IHC FCM WB

MA02236

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA02236)
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Flow cytometric Analysis of living human peripheral blood cells, using anti-CD8A antibody, compared to an IgG isotype control (A.left, B.blue) (1:25).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD8A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD8A.
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产品简介>

产品名称

Anti-CD8A Antibody (Clone#OTI7C10)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD8; CD8A; CD8a molecule; Leu2; MAL; p32

产品类型

Monoclonal

检验物种

human

应用范围

IHC, FCM, WB

基因名称

CD8A

克隆号

OTI7C10

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 22-182 of human CD8A (NP_001759) produced in E.coli.

计算分子量

27 kDa

实际分子量

23.5 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot ID

P01731

文献引用格式

CD8A Antibody (Clone#OTI7C10) (Boster Biological Technology, Wuhan, China. Catalog#MA02236)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD8 antigen, alpha chain

蛋白名全称

T-cell surface glycoprotein CD8 alpha chain

实验方案>

实验步骤

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