Anti-CD8A Antibody (Clone#CA-8)

筛选器： All FCM IHC ICC/IF

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  Flow Cytometry analysis of human PBMC cells using anti-CD8 antibody (BM0216).Overlay histogram showing human PBMC cells stained with BM0216 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD8 Antibody (BM0216,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  IHC analysis of CD8 using anti- CD8 antibody (BM0216).
  CD8 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- CD8 Antibody (BM0216) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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  Flow Cytometry analysis of human PBMC cells using anti-CD8 antibody (BM0216).Overlay histogram showing human PBMC cells stained with BM0216 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD8 Antibody (BM0216,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  IHC analysis of CD8 using anti- CD8 antibody (BM0216).
  CD8 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- CD8 Antibody (BM0216) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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